Liver proteome response of pre-harvest Atlantic salmon following exposure to elevated temperature
نویسندگان
چکیده
BACKGROUND Atlantic salmon production in Tasmania (Southern Australia) occurs near the upper limits of the species thermal tolerance. Summer water temperatures can average over 19 °C over several weeks and have negative effects on performance and health. Liver tissue exerts important metabolic functions in thermal adaptation. With the aim of identifying mechanisms underlying liver plasticity in response to chronic elevated temperature in Atlantic salmon, label-free shotgun proteomics was used to explore quantitative protein changes after 43 days of exposure to elevated temperature. RESULTS A total of 276 proteins were differentially (adjusted p-value < 0.05) expressed between the control (15 °C) and elevated (21 °C) temperature treatments. As identified by Ingenuity Pathway Analysis (IPA), transcription and translation mechanisms, protein degradation via the proteasome, and cytoskeletal components were down-regulated at elevated temperature. In contrast, an up-regulated response was identified for NRF2-mediated oxidative stress, endoplasmic reticulum stress, and amino acid degradation. The proteome response was paralleled by reduced fish condition factor and hepato-somatic index at elevated temperature. CONCLUSIONS The present study provides new evidence of the interplay among different cellular machineries in a scenario of heat-induced energy deficit and oxidative stress, and refines present understanding of how Atlantic salmon cope with chronic exposure to temperature near the upper limits of thermal tolerance.
منابع مشابه
Short-term starvation at low temperature prior to harvest does not impact the health and acute stress response of adult Atlantic salmon
A period of starvation is regarded as a sound practice in aquaculture prior to handling, transportation and harvest, to minimise impacts on welfare and ensure proper hygiene after harvest. However, documentation of welfare issues such as stress following starvation and handling in adult Atlantic salmon are lacking. This study aimed to examine gut emptying and potential stress during a two week ...
متن کاملHistopathological study of gills in experimentally amoebic gill disease (AGD) infected Atlantic salmon, Salmo salar, L.
Amoebic gill disease (AGD) is the most important parasitic disease of Atlantic salmon industry inAustralia. Atlantic salmon (Salmo salar) experimentally infected with Neoparamoeba sp. apparently showedAGD gross signs on the gill and an amoebic-associated gill pathology. Physico-chemical factors of waterduring the experiment were monitored regularly and were approximately constant (temperature: ...
متن کاملAqueous exposure to Aroclor 1254 modulates the mitogenic response of Atlantic salmon anterior kidney T-cells: indications of short- and long-term immunomodulation.
Polychlorinated biphenyls (PCBs) exist as persistent organic pollutants in numerous river systems in the United States. Unfortunately, some of these rivers are sites of active Atlantic salmon restoration programs, and polychlorinated biphenyls have been implicated as ancillary factors contributing to failed salmon restoration. Here, we investigate the immediate and chronic effects of intermedia...
متن کاملEffect of feeding rate on nutrient digestibility in Atlantic salmon, Salmo salar L.
A digestibility trial was conducted to examine the effect of feeding rate on dry matter, gross energy, crude protein and phosphorus digestibility in Atlantic salmon (Salmo salar). Duplicate groups of fish were fed 0.25, 0.5, 0.75, 1.0, 1.25 and 1.9% BW/day. The faeces were collected by Guelph-type collectors for five successive days. Dry matter, protein and phosphorus digestibility’s were all s...
متن کاملPhysiological effects of environmentally relevant, multi-day thermal stress on wild juvenile Atlantic salmon (Salmo salar)
The frequency of extreme thermal events in temperate freshwater systems is expected to increase alongside global surface temperature. The Miramichi River, located in eastern Canada, is a prominent Atlantic salmon (Salmo salar) river where water temperatures can exceed the proposed upper thermal limit for the species (~27°C). Current legislation closes the river to recreational angling when wate...
متن کامل